Protein Production and Analysis Facility


We provide variety of protein production services including cloning, expression testing and optimization, development of protein purification protocols, and in-house production of purified proteins.

Our analytical services are focused on biophysical characterization of structural and functional properties of proteins in solution, under native, non-denaturing conditions. We can perform quality control  of protein samples (folding, stability, aggregation, etc.); probe protein conformation and shape in solution; detect and characterize protein binding to proteins, peptides, small molecules and other ligands (including  determination of equilibrium (Kd) and kinetic rate (kon, koff) constants, stoichiometry, binding enthalpy and entropy).


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 PROTEIN PRODUCTION SERVICES

Expression systems:

Bacterial (E. coli)

 Insect (Baculovirus)

 Mammalian (secretion in FreeStyle 293 cells)

 

Protein production equipment (services available)

Emulsiflex-cells homogenizer (training, time)

FPLC, preparative run (service only)

 

ANALYTICAL SERVICES

 Analytical instruments (services available):

 Absorbance spectrophotometer (sample analysis, training, time)

New! Analytical ultracentrifuge/AUC (sample analysis) 

Atomic Force Microscope/AFM

 Differential scanning calorimeter/DSC (sample analysis)

 Fluorescence spectrometer/fluorimeter (sample analysis, training, time)

 FPLC, analytical run (sample analysis)

 Isothermal titration calorimeter/ITC (sample analysis, training, time)

 Stopped-flow system (sample analysis, training, time)