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Protein
Production and Analysis Facility
We provide variety of protein
production services including cloning, expression testing and optimization, development
of protein purification protocols, and in-house production of purified
proteins.
Our analytical services are focused
on biophysical characterization of structural and functional properties of
proteins in solution, under native, non-denaturing conditions. We can perform
quality control of protein samples (folding, stability, aggregation,
etc.); probe protein conformation and shape in solution; detect and characterize
protein binding to proteins, peptides, small molecules and other ligands
(including
determination of equilibrium (Kd) and kinetic rate (kon,
koff) constants, stoichiometry, binding enthalpy and entropy).
LOCATION
PERSONNEL
PRICE
LIST
FORMS
POLICIES
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PROTEIN PRODUCTION
SERVICES
Expression systems:
Bacterial (E. coli)
Insect (Baculovirus)
Mammalian (secretion in FreeStyle 293 cells)
Protein production equipment (services available)
Emulsiflex-cells homogenizer
(training, time)
FPLC,
preparative run
(service only) |
ANALYTICAL SERVICES
Analytical
instruments (services available):
Absorbance spectrophotometer (sample analysis, training, time)
New!
Analytical
ultracentrifuge/AUC (sample analysis)
Atomic
Force Microscope/AFM
Differential scanning calorimeter/DSC (sample analysis)
Fluorescence spectrometer/fluorimeter
(sample analysis, training,
time)
FPLC,
analytical run (sample analysis)
Isothermal titration calorimeter/ITC (sample analysis, training,
time)
Stopped-flow system (sample analysis, training, time)
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